Stock Yeast Solution Assignment | Homework For You
Into your labeled sterile empty test tube, collect a 5 mL sample from the stock yeast solution from BSC. Vortex regularly.
2. Clean the surface of the counting chamber with 70% ethanol solution and a Kim wipe.
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3. Place the clean cover slip on top (Most come with a specialized cover slip. Do not dispose). Do a dry run to see if you can locate the grids using the low power objective. You will be trying to locate squares 5 and 14 from Figure 3.3 on the previous page.
4. Prepare the viable stain by adding into the 500ïl methylene blue stain microfuge tube: 400ïl phosphate buffer 100ïl of your yeast culture then VORTEX. Note * Dilution Factor = __10____
5. The specially designed cover slip must be on the haemocytometer prior to loading.
6. Load the chamber by placing the pipette tip at groove (V shaped area) or slanted section on the slide and holding the pipette at a 45 angle, drop wise fill one depression. The chamber will fill by capillary action so take your time. If you overload the counting chamber the special cover slip will float: you must then disassemble, rinse and dry before starting again. Start with 15 to 20 ïl in your pipette tip, but you will not use it all; stop once the area is full.
7. There are two grids per slide, so repeat the procedure with the other side.
8. Carefully place the chamber under the microscope. Focus on the central primary square using the 10X objective. Now examine your slide under the high-dry objective (40X). Get Biology homework help today