Journal Of Cell Science Assignment | Homework For You
Outline Of Report Structure Introduction (450 WORDS) This should provide a background to the experiment as you understand it and why you have performed this particular experiment (besides it being compulsory for your course!). You should include references in this section, perhaps from text books available in the library but more likely from journal research articles. 5 references all from Journal and research article. Format is Journal of Cell Science. No plagiarism please These are the also important points : What is PCR? Different types of PCR? Restriction enzyme and how the reagent been used in PCR? Gel electrophoresis in PCR? Aims (50 words) Briefly state scientifically what you are looking for in the experiment. (No referencing in aim)
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Key points: Identify band size by using base pairs Identify DNA samples ( Positive and Negative) Identify reagents and cutting points Materials & Methods (200-250 words) 2 referencing no text book Journal and article For this practical report you can summarise the methods used in the style of a journal article and write ‘as outlined in practical notes (Spikings et al., 2015)’. Reference as Spikings et al., 2013, Manipulation of DNA, Notes, You should include information regarding any changes (intentional or unintentional) that were made to the provided protocol. Results (400-450 words) No referencing own datas The outcomes of your method.
This will include a description of the results of the PCR reaction and restriction enzyme digest as seen by agarose gel electrophoresis, including an annotated image of the gel and a figure legend. Your results section should include a description of the results, referring to the tables/figures of reagent and restriction enzyme, not just the raw data. You can show the difference between ideal data and your data. Interpret the result and labelled the figure. See journal articles for guidance as to how results can be described. Discussion (800-900 words) This is a summary and explanation of your results, where appropriate this should be related to the current scientific literature which you cite in the relevant places.
Briefly explain the rationale behind the design of primers and identify any potential weaknesses with either the primers or the PCR conditions used in our experiment. Discuss your data in the context of the information above. 5 reference no text book research journal and research articles. No plagiarism please References year between 2011-2015 recent and updated one Refer to research Journals and research articles that you can find in the library or through the LRC electronic resources. Be sure to use the Harvard system of referencing in the format requested by the Journal of Cell Science, available here:
You can also reference the schedule notes but ensure you acknowledge this i.e. Spikings et al., 2015 page X. However, it is better to and you will get more credit for using journal articles. If you use an internet source, it must be from a university or medical web site and it should be authored, not from Wikipedia or other sites with unknown authors. Please ensure that your references relate to the relevant documents and their authors/publishers etc., NOT the place where you found them. Make sure no plagiarism, deliver on time and scientific writing and follow the format of Journal Of cell science for referencing.
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Please deliver on and writing standard should be standard as I am looking for distinction. Please follow the practical protocol to understand that how practical carried out and follow the write up guideline to write the report. Please make sure follow 100% the write up guideline. Find the all attached files, 2 practical protocols, 1 write up guideline, my result picture and ideal data so in total there will be 5 files. Anything expert doesn’t understand please contact me as soon as possible. I had so many bad experiences So i don’t want them repeat. So don’t come back to me day of deadline and ask me question. So ask me before hand and please deliver on time. Thank you hope this time you guys won’t let me down. Get Biology homework help today