General Information And Laboratory Manual Assignment | Homework For You
1.Which amino acid residues (E. coli and/or maize, say which one) do you think define the active site of the enzyme?
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2.Which amino acid residues do you think define the inhibitor binding site?
3.What type of inhibitor (competitive, etc.) do you think aspartate and malate might be?
4.Do the two active sites in the maize enzyme adopt a similar conformation?
5.If the structures do represent inhibited and uninhibited structures can you explain why the active site of one might have a higher affinity than the other? (think about binding of the substrate, accessibility of the binding site)
6.(For extra bonus points) can you explain how the binding of the inhibitor changes the conformation of the active site? (Which secondary structure elements move, where and why?) Get Biology homework help today