Environmental Chemistry Assignment | Professional Essay Writers
1. Consider the excitation and emission spectra for the green fluorescent protein (GFP)collected at room temperature, a key tool in the analysis of biological systems (on next page). The excitation spectrum is the blue line and the emission spectrum is the red line.
A) Describe the difference between and absorption spectrum and an excitation spectrum.
B) Predict the relative intensity of GFP emission for excitation wavelengths of 450 nm and 480 nm.
C) Calculate the energy of the excitation and emission maxima.
D) Draw a Jablonski diagram representative of this molecule and label with quantitative. Values (this entire spectral region represents the S0→S1 transition).
E) On the same axes, sketch the excitation and emission spectra that you would expect if the protein were frozen to 4 K.
