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CDNA Accession Number Assignment | Homework For You

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November 15th, 2019

You have been allocated a cDNA accession number from the NCBI database (see table below on page 2). Your task is to design experimental strategies that will allow you to: clone the cDNA in different expression vectors and show the protein products; knock out the function of your target using CRISPR; simulate a mass spectrometry analysis of your protein using a tryptic digestion and MASCOT analysis.

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This assignment is designed to be completed throughout the semester, alongside the various lecture blocks. You should be able to perform each task immediately after you have learnt the appropriate techniques in lectures and practiced the analysis in workshops. If you leave it all until the final week of semester, you may find this assignment very, very difficult. Get Biology homework help today

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